Mitochondrial nucleoid morphology and respiratory function are altered in Drp1-deficient HeLa cells
書誌事項
- タイトル別名
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- JB Special Issue : Dynamics Regulation of Mitochondria
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<jats:title>Abstract</jats:title> <jats:p>Mitochondria are dynamic organelles that frequently divide and fuse with each other. The dynamin-related GTPase protein Drp1 has a key role in mitochondrial fission. To analyse the physiological roles of Drp1 in cultured human cells, we analysed Drp1-deficient HeLa cells established by genome editing using CRISPR/Cas9. Under fluorescent microscopy, not only mitochondria were elongated but their DNA (mtDNA) nucleoids were extremely enlarged in bulb-like mitochondrial structures (‘mito-bulbs’) in the Drp1-deficient HeLa cells. We further found that respiratory activity, as measured by oxygen consumption rates, was severely repressed in Drp1-deficient HeLa cells and that this was reversible by the co-repression of mitochondrial fusion factors. Although mtDNA copy number was not affected, several respiratory subunits were repressed in Drp1-deficient HeLa cells. These results suggest that mitochondrial fission is required for the maintenance of active respiratory activity and the morphology of mtDNA nucleoids in human cells.</jats:p>
収録刊行物
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- The journal of biochemistry
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The journal of biochemistry 167 (3), 287-294, 2020-03
Tokyo : Japanese Biochemical Society
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詳細情報 詳細情報について
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- CRID
- 1524232504525733632
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- NII論文ID
- 40022185554
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- NII書誌ID
- AA00694073
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- ISSN
- 0021924X
- 17562651
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- NDL書誌ID
- 030308181
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- 本文言語コード
- en
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- NDL 雑誌分類
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- ZR2(科学技術--生物学--生化学)
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- データソース種別
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- NDL
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