A Continuous-step Density Percoll Gradient for Use in AIH and Life Table Analysis of Pregnancy Rates

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  • SATO,Hirohisa
    Department of Obstetrics and Gynecology, Tachikawa Kyosai Hospital
  • KIMURA,Hiroyuki
    Department of Obstetrics and Gynecology, Tachikawa Kyosai Hospital
  • SAITO,Suguru
    Department of Obstetrics and Gynecology, Tachikawa Kyosai Hospital
  • HIGUCHI,Yasuhiko
    Department of Obstetrics and Gynecology, Tachikawa Kyosai Hospital
  • ENDO,Yoshihiro
    Department of Obstetrics and Gynecology, Tachikawa Kyosai Hospital
  • OHYAMA,Toshio
    Department of Obstetrics and Gynecology, Tachikawa Kyosai Hospital
  • YAMAGUCHI,Mitsuya
    Department of Obstetrics and Gynecology, Tachikawa Kyosai Hospital

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  • Percoll撹拌密度勾配法のAIH (配偶者間人工授精)への応用とLife table法による妊娠率の検討

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For use in artificial insemination with husband's semen (AIH), a continuous-step Percoll density gradient technique was used to wash and concentrate sperm from ejaculate. To evaluate usefulness, the pregnancy rates were analyzed by the life table method. 1. After washing by the continuous-step Percoll density gradient method, sperm density increased from 28.8×10^6/ml (original semen) to 40.9×10^6/ml and sperm motility improved from 52.4% (original semen) to 77.0% respectively. 2. A group of 119 infertile patients whose diagnosis involved oligoasthenozoospermia, cervical factor and unexplained infertility were selected for AIH. After 640 insemination cycles, 37 women conceived with a pregnancy rate of 31.1%. By using life table analysis, the cumulative conception probability rate reached 0.652 after 13 insemination cycles. 3. Cumulative pregnancy rates by diagnostic category were 0.494 in male factor and 0.745 in cervical factor after 12 cycles, respectively. 4. The poorest results were obtained in unexplained infertility with a cumulative pregnancy rate of only 0.355 after 12 cycles. 5. In the follow up study, 24 viable babies were born and seven (18.9%) resulted in spontaneous abortion in the first trimester. These results indicate that the continuous-step Percoll density gradient technique is useful for improving the pregnancy rate in AIH.

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