STUDIES ON CITRATE METABOLISM IN UROLITHIASIS : 1. An Enzymatic Determination of Urinary Citrate with Citrate Lyase
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- Yasukawa Shu
- Department of Urology, Izumisano Municipal Hospital
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- Takamatsu Masato
- Department of Urology, Izumisano Municipal Hospital
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- Ebisuno Shoichi
- Department of Urology, Wakayama Medical College
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- Morimoto Shigeyoshi
- Department of Urology, Wakayama Medical College
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- Yoshida Toshihiko
- Department of Urology, Wakayama Medical College
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- Ohkawa Tadashi
- Department of Urology, Wakayama Medical College
Bibliographic Information
- Other Title
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- 尿路結石症におけるクエン酸代謝の研究. 1. クエン酸リアーゼを用いた尿中クエン酸の測定
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Description
A method for enzymatic determination of urinary citrate using citrate lyase is described. The optimum pH is 7.2 and the addition of zinc ion is found to activate the reaction, but the influence of pH and zinc ion on the reaction velocity is reduced in proportion to increase of citrate lyase. The effect of the Tris HCl buffer concentration on reaction velocity is also studied, and high concentrations of the Tris HCl buffer are found to inhibit the reaction. The recovery rates of citrate added to urine ranged from 98.5% to 103% and the coefficient of variation of triplicate assay is 3.1%. The urinary citrate excretion in healthy volunteers are 397±159 and 474±209mg/day (mean±SD) for 66 males and 23 females, respectively. Since this method is highly precise and convenient, it may be readily adopted as a routine clinical examination.
Journal
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- The Japanese Journal of Urology
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The Japanese Journal of Urology 76 1848-1854, 1985
The Japanese Urological Association
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Details 詳細情報について
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- CRID
- 1570291227379250816
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- NII Article ID
- 110006531480
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- NII Book ID
- AN00196577
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- ISSN
- 00215287
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- Text Lang
- ja
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- Data Source
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- CiNii Articles
