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High efficiency transformation of fission yeast treated with thiol compounds by electroporation
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- SUGA Minoru
- Faculty of Engineering, Toyama University
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- AOYAMA Koutarou
- Faculty of Engineering, Toyama University
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- ISOBE Masaharu
- Faculty of Engineering, Toyama University
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- HATAKEYAMA Toyomasa
- Faculty of Engineering, Toyama University
Bibliographic Information
- Other Title
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- エレクトロポレーション法を用いたチオール化合物処理による分裂酵母の高効率な形質転換法
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Description
a high efficiency method of Schizosaccharomyces pombe transformation by electroporation has been developed. In pretreatment with thiol compounds before the pulse application, the cells grown on a synthetic medium (SD) instead of a natural medium (YPD) drastically improved a transformation efficiency. The transformation efficiency of this protocol was approximately 10-fold higher than that of conventional treatment without Dithiothreitol. The best efficiency, approximately 1.2×10^7 transformants per μg of DNA, was achieved in the case of electric field 11kV/cm and its time constant 5 msec.
Journal
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- IEICE technical report. ME and bio cybernetics
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IEICE technical report. ME and bio cybernetics 98 (94), 29-33, 1998-05-29
The Institute of Electronics, Information and Communication Engineers
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Keywords
Details 詳細情報について
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- CRID
- 1571980077337330432
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- NII Article ID
- 110003287424
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- NII Book ID
- AN1001320X
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- Text Lang
- ja
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- Data Source
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- CiNii Articles
