Gas Chromatography of Amino Acids:I. Analysis of the Mixture of Methyl-N-trifluoroacetyl Derivatives

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Gas chromatographic separation of a number of N-trifluoroacetyl methyl esters of amino acids was investigated using dual column programmed temperature technique.<br> Fifteen amino acids, alanine, valine, glycine, leucine, proline, aspartic acid, threonine, serine, methionine, glutamic acid, phenylalanine, hydroxyproline, lysine, tyrosine and tryptophan, can be separated on the column of 1% NGS and 1.5% SE-30 connected in series within 50 minutes.<br> The author is deeply indebted to Prof. E. Ochiai for his encoruagement. He also thanks Mr. M. Matsui and Mr. Y. Honma for their assistance in this studies.

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