Isolation of apolipoprotein A-I-containing lipoproteins by immunoaffinity chromatography using a formylated derivative of cellulose gel

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Immunoaffinity chromatography is an important tool used to purify the single component from the mixture such as plasma. In the present study, we designed a simple and efficient method for isolating lipoproteins by anti-APO A-I and Apo A-II immunoaffinity columns using Formyl-Cellufine gel. With the combination of these two columns, we quantitated two different species of Apo A-I containing lipoproteins; lipoprotein containing Apo A-I and Apo A-II (LpA-I/A-II), and lipoprotein containing Apo A-I but no Apo A-II (LpA-I). To compare the present method with the conventional one which makes use of cyanogen bromide activated agarose, we also tested anti-APO A-I and anti-APO A-II immunoaffinity columns, using a CNBr-activated agarose gel.

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