Differential effect of etretinate on proliferation and extracellular matrix metabolism of human dermal fibroblasts from elderly and young individuals in a novel three-dimensional culture
書誌事項
- 公開日
- 1998-12-01
- DOI
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- 10.1016/s0923-1811(98)00035-8
- 10.5281/zenodo.3375345
- 10.5281/zenodo.3375346
- 公開者
- Elsevier BV
この論文をさがす
説明
The effect of etretinate on proliferation and biosynthesiso f collagensa nd glycosaminoglycans(G AGS) were<br> investigatedu sing human dermal fibroblasts in a novel three-dimensionacl ulture supplementedw ith L-ascorbica cid<br> 2-phosphate. Fibroblasts from two young and two elderly individuals were studied at different concentrations of<br> etretinate, 0.25, 1. O and 2.5 pg/ml. Collagens( hydroxyproline) and GAGS (disaccharideu nits) extracted from the cell<br> layer were analyzed and quantified biochemically by high-performance liquid chromatography (HPLC). Etretinate<br> showed no significant effect on fibroblast proliferation either in the monolayer or the three-dimensionacl ulture.<br> Etretinate increasedth e collageno r GAGS content in the cell layer, which was prominent at etretinate concentrations<br> of 1.0 and 2.5 or 0.25 pg/ml, respectivelyi n fibroblastsf rom the elderly (P < 0.05). This effect was not seenin dermal<br> fibroblast from the young. Theser esultss uggesth at etretinate may have the differential effect on collagena nd GAG<br> metabolismsd ependingo n the donor age of the cultured fibroblasts.0 1998E lsevier ScienceI reland Ltd. All rights<br> reserved.
収録刊行物
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- Journal of Dermatological Science
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Journal of Dermatological Science 18 155-162, 1998-12-01
Elsevier BV
