Caffeic Acid Phenethyl Ester (CAPE) Induces VEGF Expression and Production in Rat Odontoblastic Cells

DOI IR NDL Digital Collections

Bibliographic Information

Title
Caffeic Acid Phenethyl Ester (CAPE) Induces VEGF Expression and Production in Rat Odontoblastic Cells
Other Title
  • カフェイン酸フェネチルエステル（CAPE）がラット象牙芽細胞様細胞のVEGF発現と産生に与える影響
Author
Kuramoto, Hitomi
Alias Name
  • 蔵本, 瞳
  • クラモト, ヒトミ
Author
Hirao, Kouji
Alias Name
  • 平尾, 功治
  • ヒラオ, コウジ
Author
Yumoto, Hiromichi
Alias Name
  • 湯本, 浩通
  • ユモト, ヒロミチ
Author
Hosokawa, Yuki
Alias Name
  • 細川, 由樹
  • ホソカワ, ユキ
Author
Nakanishi, Tadashi
Alias Name
  • 中西, 正
  • ナカニシ, タダシ
Author
Takegawa, Daisuke
Alias Name
  • 武川, 大輔
  • タケガワ, ダイスケ
Author
Washio, Ayako
Alias Name
  • ワシオ, アヤコ
  • ワシオ, アヤコ
Author
Kitamura, Chiaki
Alias Name
  • キタムラ, チアキ
  • キタムラ, チアキ
Author
Matsuo, Takashi
Alias Name
  • 松尾, 敬志
  • マツオ, タカシ
University
徳島大学
Types of degree
博士（歯学）
Grant ID
甲口第455号
Degree year
2020-03-23

Description

Caffeic acid phenethyl ester (CAPE), the main component of propolis, has various biological activities including anti-inflammatory effect and wound healing promotion. Odontoblasts located in the outermost layer of dental pulp play crucial roles such as production of growth factors and formation of hard tissue termed reparative dentin in host defense against dental caries. In this study, we investigated the effects of CAPE on the upregulation of vascular endothelial growth factor (VEGF) and calcification activities of odontoblasts, leading to development of novel therapy for dental pulp inflammation caused by dental caries. CAPE significantly induced mRNA expression and production of VEGF in rat clonal odontoblast-like KN-3 cells cultured in normal medium or osteogenic induction medium. CAPE treatment enhanced nuclear factor-kappa B (NF-κB) transcription factor activation, and furthermore, the specific inhibitor of NF-κB significantly reduced VEGF production. The expression of VEGF receptor- (VEGFR-) 2, not VEGFR-1, was up regulated in KN-3 cells treated with CAPE. In addition, VEGF significantly increased mineralization activity in KN-3 cells. These findings suggest that CAPE might be useful as a novel biological material for the dental pulp conservative therapy.

Keywords

Details 詳細情報について

Report a problem

Back to top