Campylobacter jejuni infection suppressed Cl⁻ secretion induced by CFTR activation in T-84 cells

DOI 機関リポジトリ NDLデジタルコレクション

書誌事項

タイトル
Campylobacter jejuni infection suppressed Cl⁻ secretion induced by CFTR activation in T-84 cells
タイトル別名
  • Campylobacter jejuni感染はT-84細胞におけるCFTR活性化によるCl⁻分泌亢進を抑制する
著者
天野, 幸恵
著者別名
  • アマノ, サチエ
  • Negoro, Sachie
著者
下畑, 隆明
著者別名
  • シモハタ, タカアキ
  • Shimohata, Takaaki
著者
ハタヤマ, ショウ
著者別名
  • Hatayama, Syo
著者
サトウ, ユリ
著者別名
  • Sato, Yuri
著者
マツモト, マリ
著者別名
  • Matsumoto, Mari
著者
イバ, ヒトミ
著者別名
  • Iba, Hitomi
著者
(中橋)粟飯原, 睦美
著者別名
  • (ナカハシ)アイハラ, ムツミ
  • Nakahashi-Aihara, Mutsumi
著者
上番増, 喬
著者別名
  • ウエバンソウ, タカシ
  • Uebansou, Takashi
著者
濵田, 康弘
著者別名
  • ハマダ, ヤスヒロ
  • Hamada, Yasuhiro
著者
ニシカワ, ヨシカズ
著者別名
  • Nishikawa, Yoshikazu
著者
ヤマサキ, シンジ
著者別名
  • Yamasaki, Shinji
著者
馬渡, 一諭
著者別名
  • マワタリ, カズアキ
  • Mawatari, Kazuaki
著者
髙橋, 章
著者別名
  • タカハシ, アキラ
  • Takahashi, Akira
学位授与大学
徳島大学
取得学位
博士（栄養学）
学位授与番号
甲栄第241号
学位授与年月日
2017-03-23

説明

Campylobacter jejuni causes foodborne disease associated with abdominal pain, gastroenteritis, and diarrhea. These symptoms are induced by bacterial adherence and invasion of host epithelial cells. C. jejuni infection can occur with a low infective dose, suggesting that C. jejuni may have evolved strategies to cope with the bacterial clearance system in the gastrointestinal tract. The mucosa layer is the first line of defense against bacteria. Mucus conditions are maintained by water and anion (especially Cl-) movement. Cystic fibrosis transmembrane conductance regulator (CFTR) is the main Cl- channel transporting Cl- to the lumen. Mutations in CFTR result in dehydrated secreted mucus and bacterial accumulation in the lungs, and recent studies suggest that closely related pathogenic bacteria also may survive in the intestine. However, the relationship between C. jejuni infection and CFTR has been little studied. Here, we used an 125I- efflux assay and measurement of short-circuit current to measure Cl- secretion in C. jejuni-infected T-84 human intestinal epithelial cells. The basic state of Cl- secretion was unchanged by C. jejuni infection, but CFTR activator was observed to induce Cl- secretion suppressed in C. jejuniinfected T-84 cells. The suppression of activated Cl- secretion was bacterial dose-dependent and duration-dependent. A similar result was observed during infection with other C. jejuni strains. The mechanism of suppression may occur by affecting water movement or mucus condition in the intestinal tract. A failure of mucus barrier function may promote bacterial adhesion or invasion of host intestinal epithelial cells, thereby causing bacterial preservation in the host intestinal tract.

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