Presence of Acetyl-CoA Synthetase in Ripened Bananas

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  • WENDAKOON Sumithra K.
    Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University
  • UEDA Yoshinori
    Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University
  • IMAHORI Yoshihiro
    Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University
  • TOMIMASU Yusuke
    Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University

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  • 黄熟バナナ果実でのアセチルCoA合成酵素の存在

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Acetyl-CoA synthetase (ACS) activity in fully ripened bananas was determined by HPLC. The ACS activity was higher for acetate as a substrate than for butyrate. The higher substrate specificity for acetate than for butyrate evidences that banana produces a great amount of acetate esters and a less amount of butyrate esters. The Km for MgCl2 for butyryl-CoA formation was 5-fold higher than that for acetyl-CoA formation. ACS activity was assayed in mitochondrial, microsomal, and cytosol fractions isolated from ripened banana pulp and the highest total enzyme activity was detected in the cytosol fraction. This is the first report on the presence of this enzyme, ACS, in fruit pulp.

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