Evaluation of Simple and Rapid Shiga Toxin Gene Detection of Enterohemorrhagic <i>Escherichia coli</i>in Food Using 3M<sup>TM</sup> Molecular Detection Assay2-STEC Gene Screen Kit

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  • 3M <sup>TM</sup>病原菌検出アッセイ2 STEC遺伝子スクリーニング<i>-stx</i>キットを用いた食品からの腸管出血性大腸菌のVT遺伝子簡易迅速検出の評価
  • 3M病原菌検出アッセイ2STEC遺伝子スクリーニング-stxキットを用いた食品からの腸管出血性大腸菌のVT遺伝子簡易迅速検出の評価
  • 3M ビョウゲンキン ケンシュツ アッセイ 2STEC イデンシ スクリーニング-stx キット オ モチイタ ショクヒン カラ ノ チョウカン シュッケツセイ ダイチョウキン ノ VT イデンシ カンイ ジンソク ケンシュツ ノ ヒョウカ

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<p>The sensitivity of the 3M TM Molecular Detection Assay 2-STEC Gene Screen (stx) assay (3M MDA2 STEC assay) was evaluated for verotoxin (VT) gene screening from food materials. The pure culture and foods such as sliced beef, tandoori paste, cucumber, etc. were used for this study. The sensitivity was obtained as 3 to 4 log CFU/mL in enrichment broth (BPW and mEC), which was cultured with food matrices. These results showed this detection kit was suitable the notification of standard methods from Ministry of health, which requires 4 log CFU/mL as detection limit in enrichment broth. This assay was useful as a rapid and simple screening method for VT gene from foods.</p>

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