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Fragmentation of DNAs of Processed Foods Made from Genetically Modified Soybeans
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- Ogasawara Takeshi
- San-Ei Gen F.F.I., Inc. Tokyo University of Agriculture and Technology
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- Arakawa Fumihiro
- San-Ei Gen F.F.I., Inc.
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- Akiyama Hiroshi
- National Institute of Health Sciences
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- Goda Yukihiro
- National Institute of Health Sciences
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- Ozeki Yoshihiro
- Tokyo University of Agriculture and Technology
Bibliographic Information
- Other Title
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- 遺伝子組換え大豆から製造された加工食品におけるDNA断片化
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Description
We studied the degradation of DNA molecules of soybean during various processes of food production. The validity of quantification of a recombinant DNA in "tofu" was confirmed using the processed food made from a mixture of non-genetically modified (GM) and pesticide-resistant (PR) soybeans. The quantitative PCR method is useful for determining the amount of PR soybeans in the foods processed by treatments at about 100℃. On the other hand, severe DNA degradation was occurred in the some kind of foods processed such as deep roast and fermentation, in which the length of DNA fragment was shorter than almost 95 bp. Therefore, in order to estimate PR soybean content in these processed foods, new probes and primers for PCR amplifying shorter than 100 bp of the DNA fragment for the target DNA will be needed.
Journal
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- Japanese Journal of Food Chemistry and Safety
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Japanese Journal of Food Chemistry and Safety 10 (3), 155-160, 2003-12-12
Japanese Society of Food Chemistry
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Details 詳細情報について
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- CRID
- 1390282680173802496
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- NII Article ID
- 110007367278
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- NII Book ID
- AA11666400
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- ISSN
- 21896445
- 13412094
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- Text Lang
- en
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- Data Source
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- JaLC
- CiNii Articles
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- Abstract License Flag
- Disallowed