Factors Affecting Color Stability of Strawberry Jam During Storage Part I. Identification and High Performance Liquid Chromatographic Determination of Strawberry Anthocyanin Pigments.

  • KIKOKU Yutaka
    Research and Development Center, Aohata Corporation
  • FUKUHARA Kimiaki
    Research and Development Center, Aohata Corporation
  • SAITO Isao
    Research and Development Center, Aohata Corporation
  • OOTA Hideaki
    Chugoku National Agricultural Experiment Station, Ministry of Agriculture, Forestry and Fisheries

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  • 加工・貯蔵条件がイチゴジャムの色安定性に及ぼす影響に関する研究 第1報 イチゴアントシアニン色素の同定と高速液体クロマトグラフィーによる分析
  • イチゴアントシアニン シキソ ノ ドウテイ ト コウソク エキタイ クロマトグ
  • Factors Affecting Color Stability of Strawberry Jam During Storage Part I
  • 加工・貯蔵条件がイチゴジャムの色安定性に及ぼす影響に関する研究(第1報)

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Anthocyanin pigments in six strawberry (Fragaria×ananassa Duch.) cultivars including America, Chiyoda, Hoko-wase, Toyonoka, Tioga and Chandler, which were used as fruit material for jam processing, were isolated by cellulose thin-layer chromatography and identified by conventional chromatographic and spectrometric techniques. Then, individual anthocyanins were quantified by high performance liquid chromatography (HPLC) using reversed C-18 column and detection at 520nm. Six strawberry cultivars contained the same five anthocyanins as follows: cyanidin-3-monoglucoside (Cy-3G), pelargonidin-3-monogalactoside (Pl-3Ga), pelargonidin-3-monoglucoside (Pl-3G), cyanidin and pelargonidin in the elution order of HPLC. Anthocyanin content of cv. Chandler was the most abundant (29.60mg/100g fresh mass) while cv. America was the smallest amount of anthocyanin (6.50mg) in the cultivars investigated. In distribution ratio of individual anthocyanin, Pl-3Ga was 85% or above in all cultivars studied. Cv. America was characterized by relative high percentage of Cy-3Ga (12%) and cv. Chandler by Pl-3G (9%). Whereas, cvs. Chiyoda, Hoko-wase and Toyonoka were characterized by relatively similar amount and ratio of anthocyanin.

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