Suppressive Effect on Tumor Metastasis by 6-(methylsulfinyl) hexyl isothiocyanates from Wasabi.

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  • FUKE Yoko
    Department of Food science & Human Nutrition, Tokyo Metropolitan College
  • SAWAKI Saeko
    Department of Food science & Human Nutrition, Tokyo Metropolitan College
  • NOMURA Takahiro
    Department of Molecular Oncology, Cancer Research Institute, Kanazawa University
  • RYOYAMA Kazuo
    Department of Bioscience and Biotechnology, Faculty of Engineering, Aomori University

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  • 実験転移腫よう系による沢ワサビ成分６‐（ｍｅｔｈｙｌｓｕｌｆｉｎｙｌ）　ｈｅｘｙｌ　ｉｓｏｔｈｉｏｃｙａｎａｔｅの転移抑制効果
  • ジッケン テンイ シュヨウケイ ニ ヨル サワ ワサビ セイブン 6 methylsulfinyl hexyl isothiocyanate ノ テンイ ヨクセイ コウカ

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Abstract

A highly metastatic Balb/c mouse cell line, r/m HM-SFME-1, is established from transformed ras/myc SFME cell line carrying active human c-Ha-ras genes. Metastasis was measured by detection of the amount of the PCR products of c-Ha-ras gene from cancer cells which had been inoculated at foot-pad of mice. A standard curve was made using r/m HM SFME-1 cell DNAs. The amount of PCR products corresponding to 123bp was measured by Bioimage Analyzer. First, we examined the suppressive effect of 6-(methylsulfinyl) hexyl isothiocyanate (6-MITC) against dissemination of tumor cells to kidney of experimental animals at day 9 of inoculation. TNP-470 which has been reported to be effective to the angiogenesis, 4-(methylsulfinyl) butyl isothiocyanate (4-MITC) from broccoli and 6-MITC were administrated to the experimental mice. The 6-MITC of wasabi was more effective than other two chemicals. Then, 6-MITC (40 or 400μM) was fed orally by mixing with drinking water to examine the suppressive effect of tumor metastasis. No visible alteration of body weight was observed by the administration of 6-MITC. All of the control mice revealed human c-Ha-ras bands. In animals fed 40μM 6-MITC, all of the lungs provided human c-Ha-ras bands. Four out of five mice administrated 400μM 6-MITC provided no detectable bands showing no metastasis, however, and only faint band was observable in one mouse lung. Thus, it was suggested that 6-MITC suppressed dissemination or metastasis of tumor cells with oral administration.

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