スケトウダラ・ミオシンＢのＮａＣｌによる変性

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タイトル別名
  • Denaturation of alaska pollack myosin B induced by NaCl.
  • スケトウダラ ミオシン B ノ NaCl ニ ヨル ヘンセイ

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Alaska pollack myosin B (MB) was dissolved in 40mM Tris-maleate (pH 7.0) containing varied concentrations (0.3-2.0M) of NaCl and stored at 10°C, to examine for physico-chemical and bio-chemical changes. At 1.0M or higher concentrations of NaCl, MB showed a marked decrease in viscosity and ATP-sensitivity, a decrease in Mg-, and EDTA-ATPase activities. These changes were accompanied by a concomitant appearance of myosin in ultracentrifugal and gel filtration patterns. Occurrence of 50mM KCl-soluble actin was also observed. The soluble actin did not activate Mg-ATPase activity nor inhibit EDTA-ATPase activity of intact myosin. On the other hand, MB quickly lost Ca-ATPase activity when treated with NaCl more than 1.0M.<br> These results indicated that most of Alaska pollack MB was dissociated into myosin and actin, and both components were denatured at higher concentration of NaCl.

収録刊行物

  • 日本水産学会誌

    日本水産学会誌 54 (8), 1429-1436, 1988

    公益社団法人 日本水産学会

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